{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300099/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300099"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Control of CD8+ T central memory cell formation via ADAP regulation of TRIM21-mediated ubiquitination of ZEB2","description":"The ADAP-SKAP1 signaling module plays a critical role in TCR-mediated activation and LFA-1-dependent adhesion; however, its regulatory function in balancing CD8+ T-cell effector differentiation, memory formation, and exhaustion remains poorly understood. Here, we demonstrated that ADAP deficiency drives CD8+ T-cell polarization toward a central memory phenotype (CD44Hi CD62LHi). During acute lymphocytic choriomeningitis virus (LCMV) Armstrong infection, Adap-/- CD8+ T cells exhibit accelerated effector differentiation, marked by elevated expression of CX3CR1, IFN-γ and granzyme B. Functional analyses revealed that ADAP-deficient CD8+ T cells display heightened cytotoxic capacity in vitro. RNA sequencing (RNA-seq) further identified ZEB2 as the most significantly upregulated transcription factor in Adap-/- CD8+ T cells. Mechanistically, ADAP interacts with the E3 ubiquitin ligase TRIM21 and positively regulates TRIM21-mediated K48-linked polyubiquitination of ZEB2, thereby impairing its binding to the SMAD3 promoter. Collectively, our findings define an ADAP-TRIM21-ZEB2 axis that governs CD8+ T-cell differentiation plasticity, offering novel insights into therapeutic strategies for modulating T-cell responses.","dates":{"publication":"2026/05/11"},"accession":"GSE300099","cross_references":{"GSM":["GSM9053690","GSM9053698","GSM9053687","GSM9053697","GSM9053686","GSM9053696","GSM9053685","GSM9053695","GSM9053694","GSM9053693","GSM9053692","GSM9053691","GSM9053704","GSM9053703","GSM9053702","GSM9053701","GSM9053700","GSM9053689","GSM9053699","GSM9053688"],"GPL":["24247"],"GSE":["300099"],"taxon":["Mus musculus"]}}