{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300205/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Rattus norvegicus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300205"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Prophylactic Lalba mRNA-LNP vaccination prevents the progression of early breast lesions","description":"We hypothesized that prophylactic vaccination targeting the potential cell-of-origin of breast cancer may contribute to the suppression of early tumorigenesis. Here, we developed lipid nanoparticle (LNP)-encapsulated mRNA vaccines expressing the alpha-lactalbumin (LALBA) protein unique to mammary luminal progenitors/alveolar-like cells. Vaccination of outbred Sprague-Dawley rats with N1-methylpseudouridine-modified or unmodified Lalba mRNA-LNP induced different degrees of LALBA-specific and non-specific immune response. While the vaccination caused no detectable toxicity in normal mammary glands or other organs, it suppressed Nitroso-N-methylurea (NMU)-induced tumorigenesis and enhanced the tumor-free survival. Single-cell transcriptomic analysis revealed that vaccination leads to the reduction of a distinct proliferative luminal progenitor population in immunoreactive early lesions. Overall, we demonstrated that prophylactic use of Lalba mRNA-LNP has the potential to suppress the initiation and progression of early breast lesions.","dates":{"publication":"2026/08/20"},"accession":"GSE300205","cross_references":{"GSM":["GSM9055760","GSM9055761","GSM9055751","GSM9055762","GSM9055763","GSM9055752","GSM9055764","GSM9055753","GSM9055754","GSM9055765","GSM9055766","GSM9055755","GSM9055767","GSM9055756","GSM9055757","GSM9055768","GSM9055758","GSM9055759"],"GPL":["34316"],"GSE":["300205"],"taxon":["Rattus norvegicus"]}}