GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300656/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300656GEOGSEfalseExpression data of neonatal brain from male intra-cytoplasmic sperm injection(ICSI)-F2 offspringVarious behavioural abnormalities were observed in the F2 offspring of ICSI (Kanatsu-Shinohara et al., 2023, J. Clin. Invest. 133(22):e170140). The data presented here aim to clarify the cause of these abnormalities, and gene expression in the neonatal brain was investigated using RNA sequencing (RNAseq). ICSI was performed using C57BL/6 epididymal sperm and oocytes. Males from the F1 generation were naturally mated with C57BL/6 females to produce the F2 generation. Gene expression in the neonatal brains of male ICSI-F2 offspring was examined. Each group contains four biological replicates.2026/04/21GSE300656GSM9065889GSM9065887GSM9065888GSM9065885GSM9065886GSM9065892GSM9065890GSM906589113112300656Mus musculus