{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300757/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300757"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Efferocytosis induces proangiogenic function and chromatin remodeling in tumor-associated macrophages","description":"Macrophages remove apoptotic cells by efferocytosis during immune-surveillance and in the tumor microenvironment (TME). However, the gradual molecular and epigenetic consequences of efferocytosis on the single macrophage are not thoroughly elucidated. Here we introduce Effero-seq, an approach that captures single-cell transcriptomic changes coupled with lysosomal acidification tracked by pHrodo, thus enabling the dissection of molecular dynamics over time following engulfment. Using apoptotic pHrodo-labeled cells as cargo, we defined the effero-score, a gene program gradually upregulated correlatively with pHrodo intensity. In a murine melanoma model, we found that efferocytosis induces a pro-angiogenic function in macrophages with spatial proximity to vascular niches. Moreover, efferocytosis reprograms chromatin accessibility, leading to attenuated macrophage response to IFNγ. In human uveal melanoma, expression of the effero-score is correlated with poor survival. Effero-seq sheds light on the molecular reprogramming induced by efferocytosis in the TME, opening new avenues for understanding macrophage function and developing targeted treatments.","dates":{"publication":"2026/06/05"},"accession":"GSE300757","cross_references":{"GSM":["GSM9068092","GSM9068091","GSM9068090","GSM9068133","GSM9068099","GSM9068132","GSM9068131","GSM9068098","GSM9068130","GSM9068097","GSM9068096","GSM9068095","GSM9068094","GSM9068093","GSM9068139","GSM9068138","GSM9068137","GSM9068136","GSM9068135","GSM9068134","GSM9068144","GSM9068143","GSM9068142","GSM9068141","GSM9068140","GSM9519131","GSM9519132","GSM9519130","GSM9519133","GSM9068049","GSM9068048","GSM9068047","GSM9068046","GSM9519110","GSM9519113","GSM9519114","GSM9519111","GSM9519112","GSM9068056","GSM9068055","GSM9068054","GSM9068053","GSM9068052","GSM9068051","GSM9068050","GSM9519117","GSM9519118","GSM9519115","GSM9519116","GSM9068059","GSM9519119","GSM9068058","GSM9068057","GSM9519120","GSM9519121","GSM9519124","GSM9519125","GSM9519122","GSM9519123","GSM9068067","GSM9068100","GSM9068066","GSM9068065","GSM9068064","GSM9068063","GSM9068062","GSM9068061","GSM9068060","GSM9068108","GSM9519128","GSM9068107","GSM9519129","GSM9068106","GSM9519126","GSM9519127","GSM9068105","GSM9068104","GSM9068103","GSM9068102","GSM9068069","GSM9068101","GSM9068068","GSM9068109","GSM9068070","GSM9068111","GSM9068078","GSM9068110","GSM9068077","GSM9068076","GSM9068075","GSM9068074","GSM9068073","GSM9068072","GSM9068071","GSM9068119","GSM9068118","GSM9068117","GSM9068116","GSM9068115","GSM9068114","GSM9068113","GSM9068079","GSM9068112","GSM9068081","GSM9068080","GSM9068122","GSM9068089","GSM9068121","GSM9068088","GSM9068087","GSM9068120","GSM9068086","GSM9068085","GSM9068084","GSM9068083","GSM9068082","GSM9519106","GSM9519107","GSM9068129","GSM9068128","GSM9068127","GSM9068126","GSM9068125","GSM9068124","GSM9519108","GSM9519109","GSM9068123"],"GPL":["19057","24247"],"GSE":["300757"],"taxon":["Mus musculus"],"PMID":["[42247485]"]}}