GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300757/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300757GEOGSEfalseEfferocytosis induces proangiogenic function and chromatin remodeling in tumor-associated macrophagesMacrophages remove apoptotic cells by efferocytosis during immune-surveillance and in the tumor microenvironment (TME). However, the gradual molecular and epigenetic consequences of efferocytosis on the single macrophage are not thoroughly elucidated. Here we introduce Effero-seq, an approach that captures single-cell transcriptomic changes coupled with lysosomal acidification tracked by pHrodo, thus enabling the dissection of molecular dynamics over time following engulfment. Using apoptotic pHrodo-labeled cells as cargo, we defined the effero-score, a gene program gradually upregulated correlatively with pHrodo intensity. In a murine melanoma model, we found that efferocytosis induces a pro-angiogenic function in macrophages with spatial proximity to vascular niches. Moreover, efferocytosis reprograms chromatin accessibility, leading to attenuated macrophage response to IFNγ. In human uveal melanoma, expression of the effero-score is correlated with poor survival. Effero-seq sheds light on the molecular reprogramming induced by efferocytosis in the TME, opening new avenues for understanding macrophage function and developing targeted treatments.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 musculus[42247485]