GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300776/primary200OKGenomicsSchizosaccharomyces pombeNon-coding RNA profiling by high throughput sequencing Expression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300776GEOGSEfalseSpliceosomal proteins direct RNA methylation to modulate gene expression and silence retrotransposons [smallRNA-seq]RNA modifications play indispensable roles in RNA processing and gene regulation, but the mechanistic diversity and transcript specificity of RNA-modifying activities remain unclear. Using genetic screening and biochemical analyses in S. pombe, we identified the RNA methyltransferase Tgs1 as a component of a protein complex called TEaM, which is specifically recruited by spliceosomal proteins to transcripts containing inefficiently spliced cryptic introns. Strikingly, the presence of cryptic introns alone can trigger TEaM-dependent silencing of stress-inducible genes and repetitive elements, including retrotransposons, while Tgs1 is also directed to gametogenic transcripts via a YTH-domain RNA-binding protein. In both contexts, Tgs1 catalyzes trimethylguanosine (TMG) capping, a modification that recruits the conserved Pir2/ARS2 protein and promotes RNA processing through the RNAi pathway. Our findings uncover how splicing machinery and YTH proteins converge on a common regulatory mechanism—TMG capping—that facilitates RNAi-mediated silencing via Pir2/ARS2, providing new insights into gene silencing pathways across eukaryotic species.2026/06/25GSE300776GSM9068375GSM9068374GSM9068373GSM9068372GSM9068371GSM9068378GSM9068377GSM906837620584300776Schizosaccharomyces pombe