{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300777/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Schizosaccharomyces pombe"," Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300777"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Spliceosomal proteins direct RNA methylation to modulate gene expression and silence retrotransposons [RNA-seq]","description":"RNA modifications play indispensable roles in RNA processing and gene regulation, but the mechanistic diversity and transcript specificity of RNA-modifying activities remain unclear. Using genetic screening and biochemical analyses in S. pombe, we identified the RNA methyltransferase Tgs1 as a component of a protein complex called TEaM, which is specifically recruited by spliceosomal proteins to transcripts containing inefficiently spliced cryptic introns. Strikingly, the presence of cryptic introns alone can trigger TEaM-dependent silencing of stress-inducible genes and repetitive elements, including retrotransposons, while Tgs1 is also directed to gametogenic transcripts via a YTH-domain RNA-binding protein. In both contexts, Tgs1 catalyzes trimethylguanosine (TMG) capping, a modification that recruits the conserved Pir2/ARS2 protein and promotes RNA processing through the RNAi pathway. Our findings uncover how splicing machinery and YTH proteins converge on a common regulatory mechanism—TMG capping—that facilitates RNAi-mediated silencing via Pir2/ARS2, providing new insights into gene silencing pathways across eukaryotic species.","dates":{"publication":"2026/06/25"},"accession":"GSE300777","cross_references":{"GSM":["GSM9682831","GSM9682832","GSM9682830","GSM9068407","GSM9068406","GSM9682828","GSM9682829","GSM9682826","GSM9682827","GSM9068379","GSM9682825","GSM9325690","GSM9068386","GSM9068385","GSM9068384","GSM9068383","GSM9325699","GSM9068382","GSM9068381","GSM9068380","GSM9325695","GSM9325696","GSM9325697","GSM9325698","GSM9325691","GSM9325692","GSM9068389","GSM9824270","GSM9824271","GSM9325693","GSM9682833","GSM9068388","GSM9682834","GSM9068387","GSM9325694","GSM9824266","GSM9824267","GSM9824268","GSM9824269","GSM9708455","GSM9708454","GSM9708451","GSM9708453","GSM9708452","GSM9068397","GSM9068396","GSM9068395","GSM9068394","GSM9068393","GSM9325688","GSM9325689","GSM9068392","GSM9068391","GSM9068390","GSM9325684","GSM9824263","GSM9325685","GSM9325686","GSM9824264","GSM9824265","GSM9325687","GSM9325681","GSM9068399","GSM9325682","GSM9325683","GSM9068398","GSM9068405","GSM9068404","GSM9068403","GSM9068402","GSM9068401","GSM9068400"],"GPL":["20584","18573"],"GSE":["300777"],"taxon":["Schizosaccharomyces pombe"," Homo sapiens"]}}