GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300777/primary200OKTranscriptomicsSchizosaccharomyces pombe Homo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300777GEOGSEfalseSpliceosomal proteins direct RNA methylation to modulate gene expression and silence retrotransposons [RNA-seq]RNA modifications play indispensable roles in RNA processing and gene regulation, but the mechanistic diversity and transcript specificity of RNA-modifying activities remain unclear. Using genetic screening and biochemical analyses in S. pombe, we identified the RNA methyltransferase Tgs1 as a component of a protein complex called TEaM, which is specifically recruited by spliceosomal proteins to transcripts containing inefficiently spliced cryptic introns. Strikingly, the presence of cryptic introns alone can trigger TEaM-dependent silencing of stress-inducible genes and repetitive elements, including retrotransposons, while Tgs1 is also directed to gametogenic transcripts via a YTH-domain RNA-binding protein. In both contexts, Tgs1 catalyzes trimethylguanosine (TMG) capping, a modification that recruits the conserved Pir2/ARS2 protein and promotes RNA processing through the RNAi pathway. Our findings uncover how splicing machinery and YTH proteins converge on a common regulatory mechanism—TMG capping—that facilitates RNAi-mediated silencing via Pir2/ARS2, providing new insights into gene silencing pathways across eukaryotic species.2026/06/25GSE300777GSM9682831GSM9682832GSM9682830GSM9068407GSM9068406GSM9682828GSM9682829GSM9682826GSM9682827GSM9068379GSM9682825GSM9325690GSM9068386GSM9068385GSM9068384GSM9068383GSM9325699GSM9068382GSM9068381GSM9068380GSM9325695GSM9325696GSM9325697GSM9325698GSM9325691GSM9325692GSM9068389GSM9824270GSM9824271GSM9325693GSM9682833GSM9068388GSM9682834GSM9068387GSM9325694GSM9824266GSM9824267GSM9824268GSM9824269GSM9708455GSM9708454GSM9708451GSM9708453GSM9708452GSM9068397GSM9068396GSM9068395GSM9068394GSM9068393GSM9325688GSM9325689GSM9068392GSM9068391GSM9068390GSM9325684GSM9824263GSM9325685GSM9325686GSM9824264GSM9824265GSM9325687GSM9325681GSM9068399GSM9325682GSM9325683GSM9068398GSM9068405GSM9068404GSM9068403GSM9068402GSM9068401GSM90684002058418573300777Schizosaccharomyces pombe Homo sapiens