{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300779/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Schizosaccharomyces pombe"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300779"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Spliceosomal proteins direct RNA methylation to modulate gene expression and silence retrotransposons [RIP-seq]","description":"RNA modifications play indispensable roles in RNA processing and gene regulation, but the mechanistic diversity and transcript specificity of RNA-modifying activities remain unclear. Using genetic screening and biochemical analyses in S. pombe, we identified the RNA methyltransferase Tgs1 as a component of a protein complex called TEaM, which is specifically recruited by spliceosomal proteins to transcripts containing inefficiently spliced cryptic introns. Strikingly, the presence of cryptic introns alone can trigger TEaM-dependent silencing of stress-inducible genes and repetitive elements, including retrotransposons, while Tgs1 is also directed to gametogenic transcripts via a YTH-domain RNA-binding protein. In both contexts, Tgs1 catalyzes trimethylguanosine (TMG) capping, a modification that recruits the conserved Pir2/ARS2 protein and promotes RNA processing through the RNAi pathway. Our findings uncover how splicing machinery and YTH proteins converge on a common regulatory mechanism—TMG capping—that facilitates RNAi-mediated silencing via Pir2/ARS2, providing new insights into gene silencing pathways across eukaryotic species.","dates":{"publication":"2026/06/25"},"accession":"GSE300779","cross_references":{"GSM":["GSM9068419","GSM9068418","GSM9068417","GSM9068439","GSM9068441","GSM9068440","GSM9068438","GSM9068416","GSM9068437","GSM9068415","GSM9068414","GSM9068436","GSM9068425","GSM9068435","GSM9068424","GSM9068413","GSM9068434","GSM9068423","GSM9068412","GSM9068422","GSM9068421","GSM9068420"],"GPL":["20584"],"GSE":["300779"],"taxon":["Schizosaccharomyces pombe"]}}