GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300942/primaryOK200GenomicsHomo sapiensNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300942GEOGSEfalseA Prospective Pilot Study of Urine microRNA as a Biomarker of Urinary Toxicity Following MRI-Guided Prostate Stereotactic Body RadiotherapyUrinary toxicities are common following prostate radiotherapy and can significantly impact quality of life. There is a need for biomarkers that can predict the risk of urinary toxicity following treatment using MRI guided stereotactic body radiotherapy (MR-SBRT) to assist with selection of the most appropriate radiotherapy treatment approach and to potentially provide early intervention for toxicities. Many studies demonstrated the applicability of miRNA from bodily fluids as biomarkers; thus, we decided to check if urine-derived miRNA can serve as radiation toxicity predictors. Both sequencing and confirmatory targeted qPCR showed that expressions of miR-26a-5p and miR-95-3p decrease during MR-SBRT and begin to increase during early follow up. Levels of miR-204-3p and miR-192-5p behave differently in patients with and without urinary toxicity. Expression of miR-375-3p is lower in patients with higher risk category at all timepoints and tends to drop during and after MR-SBRT, especially in patients with lower risk category. These results warrant further studies on urine miRNAs as non-invasively measured biomarkers.2026/09/17GSE300942GSM9072530GSM9072523GSM9072522GSM9072521GSM9072520GSM9072527GSM9072526GSM9072525GSM9072524GSM9072529GSM9072528GSM9072512GSM9072534GSM9072533GSM9072511GSM9072532GSM9072531GSM9072516GSM9072515GSM9072537GSM9072536GSM9072514GSM9072535GSM9072513GSM9072519GSM9072518GSM907251730882300942Homo sapiens