GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300965/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300965GEOGSEfalseLabile iron supply governs CD8+ T cell activation and function in metabolic health and obesitySufficient nutrient supply is critical for CD8+ T cell activation and functions, but the role of labile iron remains unclear. Here, we find a rapid increase in the supply of labile iron in CD8+ T cells upon T-cell receptor activation. Blocking labile iron supply blunts Fe2+ influx into mitochondria and ROS production, hindering CD8+ T cell activation, while excessive labile iron content causes hyperactivation. Disrupted labile iron supply impairs the maintenance of adipose tissue CD8+ T cells. In obesity, an increase in Fe2+ influx into mitochondria enhances adipose tissue CD8+ cell functions, but weight loss normalizes CD8+ T cell iron metabolism. CD8+ cell-specific NRF2 activation boosts ferritin and lowers mitochondrial Fe2+ and ROS content in adipose tissue CD8+ cells, attenuating obesity-related adipose tissue inflammation and metabolic disorders. These results highlight the crucial role of labile iron supply in CD8+ T cell activation.2026/09/23GSE300965GSM9072798GSM9072797GSM9072796GSM9072802GSM9072801GSM9072800GSM9072799GSM9072806GSM9072805GSM9072804GSM9072803GSM907280734290300965Mus musculus[41241023]