{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE301nnn/GSE301371/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Bos taurus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE301371"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptome-wide mapping of m6A in bovine oocytes and preimplantation embryos by SAC-seq [SAC-Seq]","description":"RNA N6-methyladenosine (m6A) plays a crucial role in regulating gene expression and early embryonic development. However, the m6A dynamics at single-nucleotide resolution during preimplantation development has not been characterized, and the function of m6A marks in specific transcripts remain largely unexplored. Here, by using SAC-seq, we overcome the limitation of antibody-based m6A profiling and define m6A landscapes in bovine oocytes and pre-implantation embryos at unprecedented level. Notably, we identify a specific m6A site on RPL12 (m6A-RPL12), which was highly methylated at the 8-cell stage. m6A-RPL12 deficient leads to reduced protein synthesis, disrupted expression of translation-related genes, and impaired zygotic genome activation and blastocyst formation. Supplementation with wild-type RPL12 mRNA failed to rescue the developmental arrest, indicating that m6A regulation extends beyond transcript abundance. This study provides invaluable m6A single-nucleotide resolution recourse in early embryogenesis and demonstrates that precise regulation of m6A at selected transcripts can shape early developmental outcomes in mammals.","dates":{"publication":"2026/06/10"},"accession":"GSE301371","cross_references":{"GSM":["GSM9081740","GSM9081741","GSM9081720","GSM9081742","GSM9081743","GSM9081721","GSM9081744","GSM9081722","GSM9081723","GSM9081745","GSM9081746","GSM9081724","GSM9081747","GSM9081725","GSM9081726","GSM9081748","GSM9081749","GSM9081727","GSM9081728","GSM9081729","GSM9081750","GSM9081730","GSM9081731","GSM9081732","GSM9081733","GSM9081734","GSM9081735","GSM9081736","GSM9081737","GSM9081738","GSM9081739","GSM9081719"],"GPL":["26012"],"GSE":["301371"],"taxon":["Bos taurus"]}}