GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE301nnn/GSE301371/primaryOK200TranscriptomicsBos taurusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE301371GEOGSEfalseTranscriptome-wide mapping of m6A in bovine oocytes and preimplantation embryos by SAC-seq [SAC-Seq]RNA N6-methyladenosine (m6A) plays a crucial role in regulating gene expression and early embryonic development. However, the m6A dynamics at single-nucleotide resolution during preimplantation development has not been characterized, and the function of m6A marks in specific transcripts remain largely unexplored. Here, by using SAC-seq, we overcome the limitation of antibody-based m6A profiling and define m6A landscapes in bovine oocytes and pre-implantation embryos at unprecedented level. Notably, we identify a specific m6A site on RPL12 (m6A-RPL12), which was highly methylated at the 8-cell stage. m6A-RPL12 deficient leads to reduced protein synthesis, disrupted expression of translation-related genes, and impaired zygotic genome activation and blastocyst formation. Supplementation with wild-type RPL12 mRNA failed to rescue the developmental arrest, indicating that m6A regulation extends beyond transcript abundance. This study provides invaluable m6A single-nucleotide resolution recourse in early embryogenesis and demonstrates that precise regulation of m6A at selected transcripts can shape early developmental outcomes in mammals.2026/06/10GSE301371GSM9081740GSM9081741GSM9081720GSM9081742GSM9081743GSM9081721GSM9081744GSM9081722GSM9081723GSM9081745GSM9081746GSM9081724GSM9081747GSM9081725GSM9081726GSM9081748GSM9081749GSM9081727GSM9081728GSM9081729GSM9081750GSM9081730GSM9081731GSM9081732GSM9081733GSM9081734GSM9081735GSM9081736GSM9081737GSM9081738GSM9081739GSM908171926012301371Bos taurus