GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE301nnn/GSE301384/primary200OKTranscriptomicsMus musculusExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE301384GEOGSEfalseNanostring gene expression profiling in a mouse model of antibody-mediated rejection of kidney allograftsAcute and chronic antibody mediated rejection (ABMR) continues to decrease clinical kidney graft function and survival. Dysregulated donor-specific antibody (DSA) responses are induced in B6.CCR5-/- recipients of complete MHC-mismatched A/J kidney allografts with NK cells playing a critical role in the acute ABMR. We tested the role of neutrophils in ABMR by transplanting A/J kidneys to CCR5-/- mice with a deletion in the neutrophil serine protease cathepsin G. Whereas B6.CCR5-/- recipients rejected all kidney allografts between days 18-25, 70% of allografts survived beyond day 60 in B6.CCR5-/-cG-/- recipients. At days 15-17 post-transplant DSA titers in B6.CCR5-/-cG-/- recipients were 24.3-fold higher than those in wild-type C57BL/6 allograft recipients. Allografts from B6.CCR5-/-cG-/- recipients on days 45 and 60 had typical characteristics of chronic graft injury including interstitial collagen deposition and peri-glomerular fibrosis that was accompanied by a fibrogenic transcript signature and late post-transplant production of autoantibodies to many targets, including structural proteins including collagen IV and fibronectin. Depletion of B cells at the time DSA peak titers were achieved on day 14 post-transplant decreased serum autoantibodies levels, the kidney allograft fibrogenic transcript signature, and the chronic kidney allograft injury, despite maintenance of the high DSA titers. These results indicate a critical role for neutrophil cathepsin G during acute ABMR of kidney allografts and in its absence, DSA induced late appearance of autoantibodies mediating development of chronic kidney allograft injury.2026/07/06GSE301384GSM9083246GSM9083248GSM9083247GSM9083249GSM9081920GSM9081921GSM9081922GSM9081900GSM9081923GSM9081901GSM9081924GSM9081902GSM9081925GSM9081903GSM9081904GSM9081926GSM9081927GSM9081905GSM9081928GSM9081906GSM9081907GSM9081929GSM9081908GSM9081909GSM9083251GSM9083250GSM9081896GSM9081930GSM9081897GSM9081931GSM9081898GSM9081910GSM9081899GSM9081932GSM9081911GSM9081912GSM9081913GSM9081914GSM9081915GSM9081916GSM9081917GSM9081918GSM90819193315825652301384Mus musculus