GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE301nnn/GSE301570/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE301570GEOGSEfalseEffect of Meflin overexpression on the fibroblast transcriptome in a three-dimensional culture using cell accumulation techniqueWe previously reported that Meflin, encoded by the immunoglobulin superfamily containing leucine-rich repeat (ISLR) gene, is a marker for tumor-restraining cancer-associated fibroblasts. However, the functional roles of Meflin in fibroblasts remain unclear. To investigate the functional impact of Meflin expression in fibroblasts, RNA sequencing was performed on Meflin-overexpressing NHDF-Ad cells cultured under conditions that better mimic the in vivo tumor microenvironment, using a cell accumulation technique that accurately recapitulates the fibrotic stroma observed in aggressive human cancers. Meflin overexpression resulted in the downregulation of PDPN, a well-established marker of tumor-promoting CAFs, and upregulation of DPT and COL15A1, which are markers of universal fibroblasts that resemble normal fibroblasts and are considered a potential source of diverse fibroblast subtypes.These findings suggest that high-level expression of Meflin is crucial for skewing CAFs toward a more universal fibroblast–like state.2026/01/11GSE301570GSM9085479GSM9085480GSM9085483GSM9085484GSM9085481GSM9085482GSM9085485GSM908548634284301570Homo sapiens