{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE301nnn/GSE301598/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE301598"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Comparative single-cell transcriptomic analysis of human primary and stem cell-derived islets under acute hypoxia","description":"To understand the divergent responses of different islet sources to the hypoxic stress encountered post-transplantation, we performed a direct, comparative single-cell RNA sequencing analysis of human primary islets and stem cell-derived islets (SC-islets, HUES8 line) over an acute time course (0, 6, 24, and 48 hours) of hypoxic exposure (1% O2). Our data reveal that while both islet types activate conserved core stress pathways, they subsequently execute markedly divergent adaptive strategies. Primary β-cells navigate hypoxia through a profound suppression of their mature identity and a shift towards a quiescent, energy-conserving state. In contrast, SC-β cells mount a highly plastic and metabolically dynamic response that is coupled with significant lineage instability and functional dysregulation. These findings highlight fundamental, source-specific differences in hypoxic adaptation, providing a rich resource for developing tailored strategies to improve islet transplantation success.","dates":{"publication":"2026/04/28"},"accession":"GSE301598","cross_references":{"GSM":["GSM9086269"],"GPL":["24676"],"GSE":["301598"],"taxon":["Homo sapiens"]}}