{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE301nnn/GSE301733/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE301733"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"CITEseq of hepatic CD4+ T cells from healthy mice and mice with steatohepatitis","description":"Unresolved inflammation and fibrosis are the two key features of metabolic dysfunction-associated steatohepatitis (MASH), a progressive form of steatotic liver disease that can evolve into cirrhosis and liver cancer. To better understand the role of CD4⁺ T cells in the pathogenesis of MASH, we comprehensively characterized hepatic CD4⁺ T cells in murine MASH at a single-cell protein and transcriptional level. CITE-sequencing revealed a marked shift in intrahepatic CD4⁺ T-cell composition in MASH, with enrichment of Th1, regulatory, and cytotoxic CD4⁺ T cells. Transcriptomic profiling also identified Tnfrsf4 (OX40) upregulation in hepatic CD4⁺ T cells during MASH. Together, these studies provide a proteogenomic single-cell atlas for hepatic CD4⁺ T cells and uncover a CD4⁺ T cell-dependent immunopathogenic circuit as a promising immunotherapeutic target to alleviate MASH and liver fibrosis.","dates":{"publication":"2026/05/18"},"accession":"GSE301733","cross_references":{"GSM":["GSM9088184","GSM9088185","GSM9088186","GSM9088187"],"GPL":["30172"],"GSE":["301733"],"taxon":["Mus musculus"],"PMID":["[42141897]"]}}