{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE301nnn/GSE301802/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE301802"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Peptide-based Wnt signal activation enables scalable production of clinical-grade patient-derived intestinal organoids for regenerative cell therapy","description":"Despite the clinical potential of intestinal organoids as a resource for regenerative cell therapy and bioengineering, the lack of reliable clinical-grade cultures has hampered further development. Here, we report the successful initiation and expansion of patient-derived intestinal organoids under a GMP-compliant protocol. Culture-, cost-, and time-efficiency improvements were achieved with organoid area-based passaging, one well plate culture, and the incorporation of Wnt activating peptide, PG-008. The peptide significantly enhanced organoid growth and stabilized patient-patient variability through intestinal stem cell (ISC) enrichment. Single cell RNA sequencing revealed that PG-008 resulted in remarkably pure culture consisting of ISCs and transit amplifying cells, suitable for rapid and consistent expansion. Intriguingly, our GMP-grade intestinal organoids contained LGR5+ ISCs, and injury-induced LGR5- regenerative ISCs, both enriched in peptide culture. Our study establishes clinical-grade intestinal organoids for further application, and a model facilitating in vitro investigations into regenerative stem cell induction and intestinal regeneration.","dates":{"publication":"2026/04/01"},"accession":"GSE301802","cross_references":{"GSM":["GSM9090003","GSM9090004"],"GPL":["24676"],"GSE":["301802"],"taxon":["Homo sapiens"],"PMID":["[41906165]"]}}