{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE301nnn/GSE301818/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Non-coding RNA profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE301818"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Long non-coding RNA TTN-AS1 promotes acute liver injury in sepsis: A novel potential monitoring and therapeutic target [miRNA-Seq]","description":"Sepsis-associated liver injury (SALI) is a common and severe complication of sepsis that significantly affects patient outcomes. However, reliable early biomarkers and effective therapeutic targets are still lacking. In this study, we integrated whole-blood transcriptomic sequencing data and Olink inflammation-related proteomics data from SALI patients to identify key regulatory factors. By integrating weighted gene co-expression network analysis (WGCNA), differential expression profiling, and competing endogenous RNA (ceRNA) network construction, we identified the long non-coding RNA TTN-AS1 as a potential regulatory candidate. Its upregulation in SALI was further validated using a public dataset. Functional validation in a cecal ligation and puncture (CLP) mouse model demonstrated that TTN-AS1 overexpression significantly aggravated liver injury, as evidenced by elevated serum ALT and AST levels (P < 0.001), increased inflammatory cytokines IL-6 and TNF-α (P < 0.001), disrupted liver architecture and aggravated inflammatory infiltration on H&E staining, enhanced expression of apoptosis-related proteins Caspase-3 (P < 0.001) and BAX (P < 0.01), and increased hepatocyte apoptosis indicated by TUNEL assay (P < 0.001). This is the earliest report demonstrating that lncRNA TTN-AS1 contributes to the pathogenesis of SALI, highlighting its promise as a biomarker for early diagnosis and a potential target for therapy.","dates":{"publication":"2026/07/04"},"accession":"GSE301818","cross_references":{"GSM":["GSM9090308","GSM9090309","GSM9090306","GSM9090307","GSM9090304","GSM9090305","GSM9090302","GSM9090303","GSM9090300","GSM9090301","GSM9090298","GSM9090299","GSM9090310","GSM9090296","GSM9090297","GSM9090295"],"GPL":["16791"],"GSE":["301818"],"taxon":["Homo sapiens"]}}