{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE301nnn/GSE301821/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE301821"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Long non-coding RNA TTN-AS1 promotes acute liver injury in sepsis: A novel potential monitoring and therapeutic target [RNA-Seq]","description":"Sepsis-associated liver injury (SALI) is a common and severe complication of sepsis that significantly affects patient outcomes. However, reliable early biomarkers and effective therapeutic targets are still lacking. In this study, we integrated whole-blood transcriptomic sequencing data and Olink inflammation-related proteomics data from SALI patients to identify key regulatory factors. By integrating weighted gene co-expression network analysis (WGCNA), differential expression profiling, and competing endogenous RNA (ceRNA) network construction, we identified the long non-coding RNA TTN-AS1 as a potential regulatory candidate. Its upregulation in SALI was further validated using a public dataset. Functional validation in a cecal ligation and puncture (CLP) mouse model demonstrated that TTN-AS1 overexpression significantly aggravated liver injury, as evidenced by elevated serum ALT and AST levels (P < 0.001), increased inflammatory cytokines IL-6 and TNF-α (P < 0.001), disrupted liver architecture and aggravated inflammatory infiltration on H&E staining, enhanced expression of apoptosis-related proteins Caspase-3 (P < 0.001) and BAX (P < 0.01), and increased hepatocyte apoptosis indicated by TUNEL assay (P < 0.001). This is the earliest report demonstrating that lncRNA TTN-AS1 contributes to the pathogenesis of SALI, highlighting its promise as a biomarker for early diagnosis and a potential target for therapy.","dates":{"publication":"2026/07/04"},"accession":"GSE301821","cross_references":{"GSM":["GSM9090429","GSM9090427","GSM9090428","GSM9090436","GSM9090425","GSM9090426","GSM9090437","GSM9090434","GSM9090435","GSM9090424","GSM9090432","GSM9090433","GSM9090422","GSM9090430","GSM9090420","GSM9090431"],"GPL":["24676"],"GSE":["301821"],"taxon":["Homo sapiens"]}}