GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE302nnn/GSE302127/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE302127GEOGSEfalseGalaxamide improve cisplatin-induced uterine damage via attenuating inflammation and apoptosis in in miceObjective: To evaluate the protective effects of marine cyclopeptide galaxamide against cisplatin-induced uterine toxicity in cervical cancer models, focusing on macrophage polarization and NF-κB signaling modulation ​Methods: In vivo tumor-bearing mouse models and in vitro cervical cancer cell lines were treated with cisplatin (CIS, 5 mg/kg) ± galaxamide (20 mg/kg). Uterine histopathology (hematoxylin-eosin staining), apoptosis (TUNEL assay), macrophage subsets (flow cytometry for M1/M2 markers), NF-κB activation (western blot for p65 phosphorylation), and serum cytokines (ELISA for TNF-α/IL-6) were analyzed. Embryo implantation rates were assessed to quantify endometrial receptivity ​Results: Cisplatin caused significant endometrial atrophy (↓45% thickness vs. control, P<0.01), NF-κB hyperactivation (2.8-fold p65 increase), and M1-macrophage dominance (M1/M2 ratio ↑40%). These changes correlated with elevated pro-inflammatory cytokines (TNF-α↑300%, IL-6↑180%) and reduced embryo implantation (↓67%, P<0.001). Galaxamide co-treatment: Suppressed NF-κB activation (p65↓65%, P<0.01) and apoptosis (TUNEL+ cells↓50%) Restored M2-macrophages (↑25%, P<0.05) and cytokine balance (TNF-α↓55%, IL-6↓48%) Preserved endometrial integrity and increased implantation rates (1.9-fold vs. CIS alone, P<0.01) ​Conclusion: Galaxamide mitigates cisplatin-induced uterine damage by reprogramming macrophages toward M2 phenotype and inhibiting NF-κB-mediated inflammation, offering a promising adjuvant strategy to preserve fertility in young cervical cancer patients ​Keywords: cisplatin toxicity, uterine protection, galaxamide, macrophage polarization, NF-κB pathway2026/09/01GSE302127GSM9096721GSM9096720GSM9096723GSM9096722GSM9096725GSM9096724GSM9096718GSM9096717GSM909671928430302127Mus musculus