GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE302nnn/GSE302233/primaryOK200TranscriptomicsTriticum aestivumExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE302233GEOGSEfalseThe effect of nitrogen on wheat during drought and recovery after rehydration.For transcriptomic analysis under drought, re-watering (RW), and re-watering with nitrate (RWN) conditions, wheat seedlings were germinated and transplanted into soil tanks once the first leaf emerged from the coleoptile, then irrigated with 2 L of wheat growth medium containing 5 mM nitrate, after which no further water was added. When soil moisture fell below 5%, seedlings were subjected to drought for one day before being re-watered with 2 L of medium containing either 0 mM (RW) or 5 mM (RWN) nitrate per tank. Leaf samples were collected from wheat seedlings during well-watered, drought, and at 3 hours, 1 day, and 5 days post-RW/RWN, and RNA was extracted for subsequent RNA-Seq analysis.2026/07/10GSE302233GSM9100218GSM9100229GSM9100219GSM9100230GSM9100220GSM9100231GSM9100214GSM9100236GSM9100225GSM9100226GSM9100237GSM9100215GSM9100216GSM9100227GSM9100217GSM9100228GSM9100221GSM9100232GSM9100233GSM9100222GSM9100223GSM9100234GSM9100235GSM910022434686302233Triticum aestivum