{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE302nnn/GSE302248/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE302248"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"BCAT1 as a critical regulator of human chondrosarcoma progression and differentiation through enzymatic and non-enzymatic mechanisms","description":"Human chondrosarcoma is a malignant bone tumor with a poor prognosis due to its resistance to conventional therapies. In this study, we investigated the role of Branched-Chain Amino Acid Transaminase 1 (BCAT1) in chondrosarcoma pathogenesis. BCAT1 depletion impairs proliferation and tumorigenicity both in vitro and in vivo in a xenograft model, underscoring its oncogenic role. Transcriptome analyses revealed that BCAT1 suppresses the chondrocyte differentiation pathway by inhibiting the expression of SOX9, a master regulator of normal chondrogenesis, through epigenetic regulation. This process depends on BCAT1’s enzymatic activity to generate α-ketoglutarate, which subsequently alters histone methylation status. Additionally, the CXXC motif of BCAT1 scavenges reactive oxygen species (ROS) independently of its enzymatic activity, maintaining G2/M checkpoint signaling and preventing ROS-induced cellular senescence. Finally, knockdown experiments in patient-derived primary cells confirm the clinical significance of BCAT1 function in human chondrosarcoma. These findings establish BCAT1 as a critical moonlighting enzyme in chondrosarcoma proliferation and differentiation, presenting a potential therapeutic target for this challenging cancer.","dates":{"publication":"2026/07/10"},"accession":"GSE302248","cross_references":{"GSM":["GSM9100460","GSM9100461","GSM9100462","GSM9100451","GSM9100456","GSM9100457","GSM9100458","GSM9100459","GSM9100463","GSM9100452","GSM9100453","GSM9100464","GSM9100465","GSM9100454","GSM9100455","GSM9100466"],"GPL":["18573","24676"],"GSE":["302248"],"taxon":["Homo sapiens"]}}