{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE302nnn/GSE302458/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE302458"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Generation of expandable hepatocyte organoids with multipolar organization from human embryonic stem cells","description":"In this study, we successfully generated mature grape-like heporgs (G-heporg) from human embryonic stem cells (hESCs) using a modified hepatocyte organoid culture system. Within this differentiation platform, spherical hepatocyte organoids (S-heporg) spontaneously emerged alongside G-heporgs. Similar to primary heporgs, differentiated G-heporgs exhibited high ALB expression and expansion capability, whereas the S-heporgs lacked both characteristics. Comparative analysis revealed that IGF2 stimulates G-heporg formation. Moreover, we found that targeted modulation of the Hippo-YAP pathway overcame long-term expansion limitations in G-heporgs. Crucially, the differentiated and expandable G-heporgs developed multipolar architectures with functional BC networks, recapitulating the polarity characteristic of primary hepatocytes. Finally, we modeled copper ion metabolism using G-heporgs, demonstrating their utility for investigating polarized trafficking processes of hepatocyte metabolites","dates":{"publication":"2026/04/08"},"accession":"GSE302458","cross_references":{"GSM":["GSM9104380","GSM9104391","GSM9104381","GSM9104392","GSM9104390","GSM9104388","GSM9104399","GSM9104389","GSM9104397","GSM9104386","GSM9104387","GSM9104398","GSM9104384","GSM9104395","GSM9104385","GSM9104396","GSM9104393","GSM9104382","GSM9104394","GSM9104383","GSM9104379"],"GPL":["24676"],"GSE":["302458"],"taxon":["Homo sapiens"],"PMID":["[41888105]"]}}