{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE302nnn/GSE302501/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE302501"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Posphorylation-Dependent Activation of Transcription Factors Drives Cell Fate Switching in Cortical Development","description":"Cortical development involves precise regulation of transcription factors (TFs) and signalling pathways. Here, we show that phosphorylation of TFs is a key mechanism driving cell fate transitions during neurogenesis and gliogenesis. Using multi-omics profiling of embryonic mouse cortex, we identify stage-specific phosphorylation of Hmgn3 and Nfib as critical for neuronal differentiation and maturation. Phospho-mutant analyses reveal impaired migration and astrocyte differentiation. CUT&RUN and scATAC-seq integration show phosphorylation-dependent enhancer binding by Nfib. Additionally, Hmgn3 and Nfib regulate distinct signaling pathways mediating cell-cell communication. These findings highlight phosphorylation as a central regulator of TF activity and cortical lineage specification.","dates":{"publication":"2026/07/20"},"accession":"GSE302501","cross_references":{"GSM":["GSM9105213","GSM9105211","GSM9105212","GSM9105210","GSM9105208","GSM9105209"],"GPL":["24247"],"GSE":["302501"],"taxon":["Mus musculus"]}}