GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE302nnn/GSE302501/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE302501GEOGSEfalsePosphorylation-Dependent Activation of Transcription Factors Drives Cell Fate Switching in Cortical DevelopmentCortical development involves precise regulation of transcription factors (TFs) and signalling pathways. Here, we show that phosphorylation of TFs is a key mechanism driving cell fate transitions during neurogenesis and gliogenesis. Using multi-omics profiling of embryonic mouse cortex, we identify stage-specific phosphorylation of Hmgn3 and Nfib as critical for neuronal differentiation and maturation. Phospho-mutant analyses reveal impaired migration and astrocyte differentiation. CUT&RUN and scATAC-seq integration show phosphorylation-dependent enhancer binding by Nfib. Additionally, Hmgn3 and Nfib regulate distinct signaling pathways mediating cell-cell communication. These findings highlight phosphorylation as a central regulator of TF activity and cortical lineage specification.2026/07/20GSE302501GSM9105213GSM9105211GSM9105212GSM9105210GSM9105208GSM910520924247302501Mus musculus