GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303307/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303307GEOGSEfalseSuper-enhancer-driven TCF4 promotes neuroblastoma metastasis by mediating GM3 synthesis [RNA-Seq]Metastasis remains a critical factor for survival in neuroblastoma (NB). Although NB metastasis involves multifaceted regulatory mechanisms, the role of epitranscriptomic regulation in this process has not been elucidated. Here, we identify super-enhancer-driven transcription factor TCF4 as a central orchestrator of metastatic networks in NB, promoting both in vitro and in vivo dissemination. Mechanistically, TCF4 transcriptionally activates SPTLC1, a pivotal enzyme in sphingolipid biosynthesis, to promote ganglioside GM3 synthesis. GM3 orchestrates membrane architecture remodeling, thereby modulating ITGB1 membrame localization and activation, which subsequently potentiates metastasis-associated FAK signaling. Notably, we demonstrate that the HDAC6 inhibitor ACY-1215 suppresses NB malignancy by destabilizing TCF4 protein. These findings reveal an epitranscriptomic-metabolic axis governing NB metastasis and propose ACY-1215 as a translational therapeutic candidate for clinical intervention.2026/05/27GSE303307GSM9123111GSM9123114GSM9123115GSM9123112GSM9123113GSM9123118GSM9123116GSM912311729480303307Homo sapiens[41630015]