GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303494/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303494GEOGSEfalseIncreased constitutive interferon-β levels and altered CD4 T cell homeostasis induced by expression of a viral glycoproteinBesides the strong type I interferon (IFN-I) response induced by infections, IFN-I is also produced constitutively at lower levels. Constitutive IFN-I production is often regulated by resident microbiota and is essential for induction of efficient immune responses. Here, we demonstrate in transgenic (tg) mice that expression of a single viral envelope gene, the glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV), increased constitutive IFN-β levels and induced numerous IFN-stimulated genes (ISGs). In addition, self-antigen-driven CD44highCD62Llow memory-phenotype and regulatory CD4 T cell populations were enlarged in these LCMV GP-tg mice but bona fide antigen-specific memory CD4 T cells were unchanged. In conclusion, our study shows that increased constitutive IFN-β levels induced by expression of a single viral envelope gene and accompanied by upregulated ISGs significantly altered CD4 T cell homeostasis. Furthermore, our data imply that a pathway capable of upregulating constitutive IFN-β levels can sense LCMV GP expression in vivo.2026/04/02GSE303494GSM9128069GSM9128072GSM9128073GSM9128074GSM9128070GSM912807124247303494Mus musculus[41972454]