GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303528/primary200OKTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303528GEOGSEfalseRNA Sequencing Reveals Differential Gene Expression in Human Brain Microvascular Endothelial Cells (HBMECs) Subjected to Oxygen-Glucose Deprivation/Reperfusion (OGD/R)Human brain microvascular endothelial cells (HBMECs) are a critical component of the blood-brain barrier (BBB), and their stability is paramount for BBB integrity, particularly under pathological stress such as ischemic stroke. This study employed an *in vitro* model of ischemia-reperfusion injury using oxygen-glucose deprivation followed by reperfusion (OGD/R) on cultured HBMECs. To comprehensively characterize the transcriptional response, we performed high-throughput RNA sequencing (RNA-seq) on both OGD/R-treated and control (normoxic) HBMEC samples. Our analysis identified a significant number of differentially expressed genes (DEGs) associated with the OGD/R challenge. These DEGs encompass genes involved in key biological processes pertinent to endothelial cell dysfunction, BBB disruption, inflammatory responses, oxidative stress, apoptosis, and potential protective/repair mechanisms. This dataset provides a detailed transcriptional profile of HBMECs undergoing simulated ischemia-reperfusion injury. It serves as a valuable resource for elucidating the molecular mechanisms underlying BBB compromise in ischemic stroke and for identifying novel potential therapeutic targets aimed at preserving BBB function and mitigating neuronal damage.2026/06/30GSE303528GSM9128531GSM9128532GSM9128533GSM9128534GSM9128535GSM912853624676303528Homo sapiens