GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303559/primaryOK200TranscriptomicsHomo sapiens Non-coding RNA profiling by high throughput sequencingExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303559GEOGSEfalseA functional antagonism between let-7 and miR-17 family members relying on sequences outside the seedThe canonical function of miRNAs relies on their ability to recognize and target messenger RNAs (mRNAs) through a mechanism known as seed pairing. The seed is a conserved sequence located at the 5'-end of the miRNA, and its specific pairing with the target mRNA is essential for target regulation. Surprisingly, we observed that miR-106a can bind to targets in place of let-7a despite the entirely different seed sequences. This binding does not depend on the seed sequence of miR-106a but instead relies on the extensive pairing on its 3'-end that shows homology with the let-7a seed sequence. miR-106a binding does not induce direct regulation of the bound targets. It shields them from seed-mediated pairing with let-7 family members, leading to competition for pairing with the same sequence in the target mRNA and resulting in two opposing functional outcomes: target repression by let-7a or target shielding by miR-106a. We conclude that by preventing let -7a from repressing oncogenic targets, the noncanonical binding of miR-106a contributes to cancer development.2026/08/14GSE303559GSM9130679GSM9130677GSM9130678GSM9130686GSM9130687GSM9130684GSM9130685GSM9130682GSM9130683GSM9130680GSM9130681GSM9130688GSM9130689GSM9130697GSM9130698GSM9130610GSM9130695GSM9130696GSM9130693GSM9130694GSM9130691GSM9130692GSM9130690GSM9130619GSM9130617GSM9130618GSM9130615GSM9130616GSM9130613GSM9130614GSM9130611GSM9130699GSM9130612GSM9130620GSM9130621GSM9130628GSM9130629GSM9130626GSM9130627GSM9130624GSM9130625GSM9130622GSM9130623GSM9130631GSM9130632GSM9130630GSM9130639GSM9130637GSM9130638GSM9130635GSM9130636GSM9130633GSM9130634GSM9130642GSM9130643GSM9130640GSM9130641GSM9640524GSM9640523GSM9640526GSM9640525GSM9640522GSM9640521GSM9130648GSM9130649GSM9130646GSM9130647GSM9130644GSM9130645GSM9130653GSM9130654GSM9130651GSM9130652GSM9130650GSM9130659GSM9130657GSM9130658GSM9130655GSM9130656GSM9130664GSM9130665GSM9130662GSM9130663GSM9130660GSM9130661GSM9130705GSM9130703GSM9130704GSM9130701GSM9130668GSM9130669GSM9130702GSM9130666GSM9130667GSM9130700GSM9130675GSM9130676GSM9130673GSM9130674GSM9130671GSM9130672GSM913067018573303559Homo sapiens[42598859]