{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303727/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":[" Genome binding/occupancy profiling by high throughput sequencing","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303727"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Toward a more precise immunophenotypic and molecular definition of human cMoPs","description":"Monocytes arise from haematopoietic progenitors in the bone marrow and play crucial roles in both homeostasis and inflammation. Human common monocyte progenitors (cMoPs) were originally identified as CLEC12A⁺CD64hi cells but have recently been redefined as CD115⁺CD64hi/int cells. We therefore evaluated whether CLEC12A⁺CD64hi cMoPs and CD115⁺CD64hi/int cMoPs represent the same cell type using flow cytometry, scRNA-seq, morphological, and functional analyses. We demonstrate that including CD115 in the gating strategy, rather than CLEC12A, yields a more accurate definition of cMoPs. Differentiation assays demonstrate the transition of CD115⁺CD64int cMoPs into CD115⁺CD64hi cMoPs and a comparable capacity for the two cMoPs to generate monocyte progeny. scRNA-seq clustering of the CD115⁺CD64hi/int cMoPs identified five different transcriptional features, including maturation-associated programs, IFN response and stress-related genes. ATAC-seq analysis of CD115⁺CD64hi/int cMoPs identified IRF8, PU.1, and also C/EBPδ as transcription factors putatively involved in monocyte development. Based on a more accurate definition and analysis, our data demonstrates the molecular complexity of human cMoPs.","dates":{"publication":"2026/08/18"},"accession":"GSE303727","cross_references":{"GSM":["GSM9552535","GSM9552534","GSM9552533","GSM9552532","GSM9552531","GSM9552530","GSM9552529","GSM9552528","GSM9134800","GSM9552527"],"GPL":["18573","11154"],"GSE":["303727"],"taxon":["Homo sapiens"]}}