{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303756/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303756"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Loss of tolerance in cow milk allergic endotypes is linked to allergen-specific CD4+ T cell subset outgrowth and microbial dysbioses","description":"Cow milk allergy (CMA) is the most prevalent pediatric food allergy, presenting in IgE and non-IgE mediated forms. Currently, the CD4+ T cell inflammatory responses underlying non-IgE CMA remain poorly characterized. By applying an allergen-reactive CD4+ T cell sorting and differential gene expression analysis approach, we characterized the CD4+ T cell subsets associated with both IgE and non-IgE mediated CMA endotypes and assessed their associations with gut bacterial compositions. Analysis of conventional (Tcon) and regulatory (Treg) CD4+ T cells revealed an expansion of inflammatory Tcon in CMA subjects, coincident with TH-like subset polarization and alterations in Treg signaling pathways. TH2-skewing was dominant in IgE mediated allergies, whereas subjects with non-IgE mediated allergies exhibited a CD4+ T cell compartment dominated by a novel TH response. Microbial dysbiosis was observed across all CMA endotypes, and network mapping revealed significant associations between a decrease in butyrate producing bacteria and the presence of inflammatory TH-like subsets. These insights link the composition of the allergen-reactive Tcon/Treg compartment to CMA pathology and identifies differences in bacterial dysbioses associated with specific disease endotypes.","dates":{"publication":"2026/07/27"},"accession":"GSE303756","cross_references":{"GSM":["GSM9135293","GSM9135292","GSM9135295","GSM9135294","GSM9135330","GSM9135297","GSM9135296","GSM9135332","GSM9135299","GSM9135298","GSM9135331","GSM9135334","GSM9135333","GSM9135336","GSM9135335","GSM9135338","GSM9135337","GSM9135339","GSM9135291","GSM9135290","GSM9135341","GSM9135340","GSM9135343","GSM9135342","GSM9135345","GSM9135344","GSM9135347","GSM9135346","GSM9135349","GSM9135348","GSM9135350","GSM9135352","GSM9135351","GSM9135354","GSM9135353","GSM9135356","GSM9135355","GSM9135358","GSM9135357","GSM9135359","GSM9135361","GSM9135360","GSM9135363","GSM9135362","GSM9135365","GSM9135364","GSM9135367","GSM9135366","GSM9135369","GSM9135368","GSM9135370","GSM9135372","GSM9135371","GSM9135374","GSM9135373","GSM9135376","GSM9135375","GSM9135378","GSM9135377","GSM9135379","GSM9135381","GSM9135380","GSM9135383","GSM9135382","GSM9135385","GSM9135384","GSM9135387","GSM9135386","GSM9135301","GSM9135389","GSM9135388","GSM9135300","GSM9135303","GSM9135302","GSM9135305","GSM9135304","GSM9135307","GSM9135306","GSM9135392","GSM9135391","GSM9135394","GSM9135393","GSM9135310","GSM9135312","GSM9135311","GSM9135314","GSM9135313","GSM9135316","GSM9135315","GSM9135318","GSM9135317","GSM9135309","GSM9135308","GSM9135390","GSM9135282","GSM9135281","GSM9135284","GSM9135283","GSM9135286","GSM9135285","GSM9135321","GSM9135288","GSM9135287","GSM9135320","GSM9135323","GSM9135289","GSM9135322","GSM9135325","GSM9135324","GSM9135327","GSM9135326","GSM9135329","GSM9135328","GSM9135319"],"GPL":["24676"],"GSE":["303756"],"taxon":["Homo sapiens"]}}