GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE304nnn/GSE304012/primaryOK200TranscriptomicsDanio rerioExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE304012GEOGSEfalseOxidation Polyethylene Microplastics Disrupt Lipid Transport in Aquatic Models: A Comparative Study Using Planktonic Crustacean and Zebrafish EmbryoEnvironmental aging processes, such as oxidation, can significantly alter the physicochemical properties and toxicity profiles of microplastics (MPs). Here, we investigated the toxicological impact of pristine polyethylene (PE) and oxidized polyethylene (OPE) microplastics using a dual-species aquatic model comprising Daphnia magna and zebrafish embryos. Characterization confirmed OPE particles exhibited increased surface roughness, higher surface charge, and broader size distribution compared to PE. Exposure to OPE induced pronounced lipid accumulation and reduced heart rate in both models. Transcriptomic analysis revealed that OPE suppressed key lipid transport and metabolism genes, including mttp, apoea, and apobb. These effects were validated through qPCR and lipid staining techniques. Notably, zebrafish embryos exposed to OPE showed significant developmental impairment even with intact chorions, suggesting enhanced bioavailability of oxidized particles. This study highlights the amplified toxicity of aged microplastics and underscores the importance of evaluating environmentally relevant forms when assessing the ecological risk of MPs.2026/07/01GSE304012GSM9140966GSM9140967GSM9140968GSM9140969GSM9140964GSM914096524995304012Danio rerio[42252446]