GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE304nnn/GSE304109/primary200OKTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE304109GEOGSEfalseRNA-seq profiling of SEM B-ALL cells silenced for KMT2A::AFF1 fusion circular RNA.The t(4;11) translocation, resulting in the KMT2A::AFF1 fusion gene, is associated with a poor prognosis in pediatric and adult B-cell Acute Lymphoblastic Leukemia (B-ALL). Recently, KMT2A::AFF1 fusion circular RNAs (f-circRNAs) were identified by bioinformatics in B-ALL; however, their contribution to leukemia has not been explored. In this context, we identified a novel recurrent back-splicing junction joining the AFF1 exon 8 to KMT2A exon 2 (AK_8_2), which was recurrent in t(4;11)-positive B-ALL cell lines as well as in pediatric and adult B-ALL patients. To investigate its functional role, we performed the total RNA-seq profiling of SEM B-ALL cells harboring the t(4;11) translocation and knocked-down for AK_8_2 f-circRNA, thus identifying a set of up-regulated genes potentially relevant for cell metabolism and leukemogenesis.2026/07/30GSE304109GSM9143721GSM9143722GSM9143720GSM9143719GSM9143723GSM914372430173304109Homo sapiens