{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE304nnn/GSE304232/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE304232"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Human DCM-time machine unravels cell state changes during primitive gut tube differentiation [RNA-seq]","description":"Cell state changes in development and differentiation are directed by gene and enhancer activity dynamics, which are difficult to study in real time. We introduce the DCM-time machine (DCM-TM) into human induced pluripotent stem cells (iPSCs) to enable retrospective, genome-wide tracing of transcriptional activity. This system labels active genes and enhancers with DCM methylation marks that are propagated during S-phase. Applied to iPSC differentiation towards definitive endoderm and primitive gut tube, DCM-TM resolves activity dynamics and enables integration with transcription factor networks and CpG methylation profiles. Together, DCM-TM provides a powerful platform for dissecting both healthy and pathogenic embryogenesis.","dates":{"publication":"2026/09/11"},"accession":"GSE304232","cross_references":{"GSM":["GSM9145586","GSM9145585","GSM9145584","GSM9145583","GSM9145582","GSM9145581"],"GPL":["30173"],"GSE":["304232"],"taxon":["Homo sapiens"]}}