{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE304nnn/GSE304236/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE304236"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Human DCM-time machine unravels cell state changes during primitive gut tube differentiation [MeD-seq]","description":"Cell state changes in development and differentiation are directed by gene and enhancer activity dynamics, which are difficult to study in real time. We introduce the DCM-time machine (DCM-TM) into human induced pluripotent stem cells (iPSCs) to enable retrospective, genome-wide tracing of transcriptional activity. This system labels active genes and enhancers with DCM methylation marks that are propagated during S-phase. Applied to iPSC differentiation towards definitive endoderm and primitive gut tube, DCM-TM resolves activity dynamics and enables integration with transcription factor networks and CpG methylation profiles. Together, DCM-TM provides a powerful platform for dissecting both healthy and pathogenic embryogenesis.","dates":{"publication":"2026/09/11"},"accession":"GSE304236","cross_references":{"GSM":["GSM9145614","GSM9145613","GSM9145612","GSM9145611","GSM9145599","GSM9145610","GSM9145598","GSM9145597","GSM9145596","GSM9145595","GSM9145594","GSM9145609","GSM9145608","GSM9145607","GSM9145606","GSM9145605","GSM9145626","GSM9145604","GSM9145603","GSM9145625","GSM9145624","GSM9145602","GSM9145623","GSM9145601","GSM9145600","GSM9145622","GSM9145621","GSM9145620","GSM9145619","GSM9145618","GSM9145617","GSM9145616","GSM9145615"],"GPL":["30173"],"GSE":["304236"],"taxon":["Homo sapiens"]}}