GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE304nnn/GSE304236/primary200OKOtherHomo sapiensOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE304236GEOGSEfalseHuman DCM-time machine unravels cell state changes during primitive gut tube differentiation [MeD-seq]Cell state changes in development and differentiation are directed by gene and enhancer activity dynamics, which are difficult to study in real time. We introduce the DCM-time machine (DCM-TM) into human induced pluripotent stem cells (iPSCs) to enable retrospective, genome-wide tracing of transcriptional activity. This system labels active genes and enhancers with DCM methylation marks that are propagated during S-phase. Applied to iPSC differentiation towards definitive endoderm and primitive gut tube, DCM-TM resolves activity dynamics and enables integration with transcription factor networks and CpG methylation profiles. Together, DCM-TM provides a powerful platform for dissecting both healthy and pathogenic embryogenesis.2026/09/11GSE304236GSM9145614GSM9145613GSM9145612GSM9145611GSM9145599GSM9145610GSM9145598GSM9145597GSM9145596GSM9145595GSM9145594GSM9145609GSM9145608GSM9145607GSM9145606GSM9145605GSM9145626GSM9145604GSM9145603GSM9145625GSM9145624GSM9145602GSM9145623GSM9145601GSM9145600GSM9145622GSM9145621GSM9145620GSM9145619GSM9145618GSM9145617GSM9145616GSM914561530173304236Homo sapiens