GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE304nnn/GSE304292/primaryOK200GenomicsBos taurusNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE304292GEOGSEfalseOxidative stress alters miRNA content in stressed granulosa cells and their derived extracellular vesiclesGranulosa cells (GCs) play a central role in oocyte maturation and follicular development, and their function is very sensitive to oxidative stress, which can compromise female fertility. Extracellular vesicles (EVs) released by GCs serve as intercellular messengers and are known to carry bioactive molecules, including microRNAs (miRNAs), which can modulate stress responses and cellular communication within the follicular environment. This study aimed to characterize and compare the miRNA profiles of both GCs and their secreted EVs following exposure to oxidative stress. Bovine GCs were treated with 5 µM H₂O₂ for 40 min, followed by 24 hours of culture. EVs were isolated from the conditioned media using size-exclusion chromatography and characterized by nanoparticle tracking analysis, transmission electron microscopy, and flow cytometry. Small RNA sequencing was performed on both the cells and their secreted EVs. Exposure to oxidative stress increased intracellular ROS levels and decreased mitochondrial activity in GCs. Differential expression analysis revealed no significant changes in miRNA abundance in GC in response to oxidative stress. In GC derived EVs, however, 10 miRNAs were significantly higher abundant, including miR-134, miR-10175-5p, miR-197, miR-2284h-5p, miR-2284y, miR-2285av, miR-2285au, miR-369-5p, miR2411-5p and miR-2387. Functional enrichment analysis of predicted targets for EV-miRNAs revealed involvement in processes such as angiogenesis, cell migration as well as WNT, MAPK and Oxitocin signaling. These findings suggest that oxidative stress alters the miRNA cargo of EVs, potentially affecting intra-follicular communication and stress adaptation. This dual characterization provides new insights into the regulatory role of EV-derived miRNAs in ovarian physiology under oxidative conditions.2026/05/15GSE304292GSM9146857GSM9146856GSM9146855GSM9146854GSM9146853GSM9146852GSM9146851GSM9146850GSM9146860GSM9146849GSM9146859GSM914685826012304292Bos taurus[41882814]