GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE304nnn/GSE304323/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE304323GEOGSEfalseOligodendrocyte Piezo2 is a regulator of age-dependent myelin integrity and dysregulated in multiple sclerosis - ON snRNA-seq dataTriggered by the relevance of Piezo mechanosensitive ion channels in different organs with functions beyond touch and pain sensation, we aimed to investigate Piezo2 in the central nervous system with a focus on the anterior visual system, especially because its role in the CNS lacks understanding. We found a bimodal expression pattern with Piezo1 in progenitors and Piezo2 in mature oligodendrocytes, which was conserved between mice and humans. Using two different OL-specific loss-of-function approaches, we found Piezo2 to be relevant for myelin compactness and white matter integrity in aged mice. Ultrastructural imaging was paired with snRNA-seq analysis and motor function testing that confirmed regulatory roles of Piezo2 in motor function and white matter integrity. We complemented our work with a validation study in human control and multiple sclerosis optic nerve tissue, and found Piezo2 to be downregulated in OLs towards the lesion rim.2026/06/03GSE304323GSM9147393GSM9147394GSM9147395GSM9147396GSM9147397GSM9147398GSM9147399GSM914740024247304323Mus musculus[42323468]