{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305149/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305149"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Sustained AMPAR expression in CA3 neurons may mediate neuroprotection following FLASH-RT","description":"To elucidate the early mechanisms underlying the long-term neuroprotective effect of FLASH-RT in the normal brain, spatial transcriptomics (Nanostring) were performed after whole-brain irradiation of C57BL/6J mice with either 1 or 3 fractions of 10 Gy at 5.6 × 106 Gy/s (1 pulse-FLASH) or at conventional dose-rate 0.1 Gy/s. FLASH-RT induced a distinct transcriptomic signature in the cornu ammonis region 3 (CA3) and dentate gyrus (DG) neurons, with upregulation of genes encoding glutamate receptors involved in calcium signaling, long-term potentiation, and mitochondrial OXPHOS. Early transcriptional upregulation of Gria genes translated into increased AMPAR protein levels at 48 h in the DG and CA3 region and sustained higher AMPAR expression at 2 and 4 weeks post-FLASH. These findings support a durable activation of AMPAR. We propose a mechanism to explain FLASH-induced neuroprotection initiated by early calcium influx and subsequent sustained expression of glutamate AMPARs in neurons and/or neural progenitors of the CA3, potentially contributing to long-term cognitive sparing.","dates":{"publication":"2026/07/07"},"accession":"GSE305149","cross_references":{"GSM":["GSM9163088","GSM9163089","GSM9163086","GSM9163087","GSM9163080","GSM9163081","GSM9163084","GSM9163085","GSM9163082","GSM9163083","GSM9163077","GSM9163078","GSM9163075","GSM9163076","GSM9163079","GSM9163070","GSM9163073","GSM9163074","GSM9163071","GSM9163072","GSM9162898","GSM9162899","GSM9162896","GSM9162897","GSM9163099","GSM9163097","GSM9163098","GSM9163091","GSM9163092","GSM9163090","GSM9163095","GSM9163096","GSM9163093","GSM9163094","GSM9162906","GSM9162907","GSM9162904","GSM9162905","GSM9162908","GSM9162909","GSM9162902","GSM9162903","GSM9162900","GSM9162901","GSM9163100","GSM9163103","GSM9163104","GSM9163101","GSM9163102","GSM9162928","GSM9162929","GSM9162926","GSM9162927","GSM9162920","GSM9162921","GSM9162924","GSM9162925","GSM9162922","GSM9162923","GSM9162917","GSM9162918","GSM9162915","GSM9162916","GSM9162919","GSM9162910","GSM9162913","GSM9162914","GSM9162911","GSM9162912","GSM9163000","GSM9163121","GSM9163001","GSM9163122","GSM9163120","GSM9163004","GSM9163125","GSM9163126","GSM9162950","GSM9163005","GSM9163123","GSM9163002","GSM9163003","GSM9163124","GSM9162948","GSM9162949","GSM9163118","GSM9162942","GSM9162943","GSM9163119","GSM9162940","GSM9163116","GSM9163117","GSM9162941","GSM9162946","GSM9162947","GSM9162944","GSM9162945","GSM9163110","GSM9163111","GSM9163114","GSM9163115","GSM9163112","GSM9163113","GSM9162939","GSM9162937","GSM9162938","GSM9162931","GSM9163107","GSM9162932","GSM9163108","GSM9163105","GSM9163106","GSM9162930","GSM9162935","GSM9162936","GSM9163109","GSM9162933","GSM9162934","GSM9163143","GSM9163022","GSM9163023","GSM9163144","GSM9163020","GSM9163141","GSM9163021","GSM9163142","GSM9162971","GSM9163026","GSM9163147","GSM9162972","GSM9163027","GSM9163148","GSM9163024","GSM9163145","GSM9163146","GSM9162970","GSM9163025","GSM9163140","GSM9162964","GSM9163019","GSM9162965","GSM9163017","GSM9163138","GSM9162962","GSM9163018","GSM9162963","GSM9163139","GSM9162968","GSM9162969","GSM9162966","GSM9162967","GSM9163011","GSM9163132","GSM9163012","GSM9163133","GSM9163130","GSM9163010","GSM9163131","GSM9163015","GSM9163136","GSM9163137","GSM9163016","GSM9163134","GSM9163013","GSM9163014","GSM9163135","GSM9162959","GSM9163129","GSM9163008","GSM9162953","GSM9163009","GSM9162951","GSM9163006","GSM9163127","GSM9162952","GSM9163007","GSM9163128","GSM9162956","GSM9163044","GSM9163165","GSM9163166","GSM9162990","GSM9163045","GSM9163163","GSM9163042","GSM9163043","GSM9163164","GSM9163169","GSM9163048","GSM9162993","GSM9162994","GSM9163049","GSM9162991","GSM9163046","GSM9163167","GSM9162992","GSM9163047","GSM9163168","GSM9163040","GSM9163161","GSM9163041","GSM9163162","GSM9163160","GSM9162986","GSM9162987","GSM9162984","GSM9163039","GSM9162985","GSM9162988","GSM9162989","GSM9163033","GSM9163154","GSM9163034","GSM9163155","GSM9163031","GSM9163152","GSM9163032","GSM9163153","GSM9163037","GSM9163158","GSM9162982","GSM9163038","GSM9162983","GSM9163159","GSM9162980","GSM9163035","GSM9163156","GSM9163157","GSM9162981","GSM9163036","GSM9163150","GSM9163030","GSM9163151","GSM9162975","GSM9162976","GSM9163149","GSM9163028","GSM9162973","GSM9162974","GSM9163029","GSM9162979","GSM9162977","GSM9162978","GSM9163066","GSM9162890","GSM9162891","GSM9163067","GSM9163064","GSM9163065","GSM9162894","GSM9162895","GSM9162892","GSM9163068","GSM9163069","GSM9162893","GSM9163062","GSM9163063","GSM9163060","GSM9163061","GSM9162887","GSM9162888","GSM9162885","GSM9162886","GSM9162889","GSM9163055","GSM9163056","GSM9163053","GSM9163174","GSM9163054","GSM9163059","GSM9162884","GSM9163057","GSM9163058","GSM9163051","GSM9163172","GSM9163052","GSM9163173","GSM9163170","GSM9163050","GSM9163171","GSM9162997","GSM9162998","GSM9162995","GSM9162996","GSM9162999"],"GPL":["24247"],"GSE":["305149"],"taxon":["Mus musculus"]}}