GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305149/primaryOK200OtherMus musculusOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305149GEOGSEfalseSustained AMPAR expression in CA3 neurons may mediate neuroprotection following FLASH-RTTo elucidate the early mechanisms underlying the long-term neuroprotective effect of FLASH-RT in the normal brain, spatial transcriptomics (Nanostring) were performed after whole-brain irradiation of C57BL/6J mice with either 1 or 3 fractions of 10 Gy at 5.6 × 106 Gy/s (1 pulse-FLASH) or at conventional dose-rate 0.1 Gy/s. FLASH-RT induced a distinct transcriptomic signature in the cornu ammonis region 3 (CA3) and dentate gyrus (DG) neurons, with upregulation of genes encoding glutamate receptors involved in calcium signaling, long-term potentiation, and mitochondrial OXPHOS. Early transcriptional upregulation of Gria genes translated into increased AMPAR protein levels at 48 h in the DG and CA3 region and sustained higher AMPAR expression at 2 and 4 weeks post-FLASH. These findings support a durable activation of AMPAR. We propose a mechanism to explain FLASH-induced neuroprotection initiated by early calcium influx and subsequent sustained expression of glutamate AMPARs in neurons and/or neural progenitors of the CA3, potentially contributing to long-term cognitive sparing.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 musculus