{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305196/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Marchantia polymorpha"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305196"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Two evolutionarily conserved fatty acid desaturases control the production of omega-3 fatty acids and bioactive jasmonates in Marchantia polymorpha","description":"In the model bryophyte Marchantia polymorpha, omega-3 (n3) polyunsaturated fatty acids (n3-PUFAs) and long-chain n3-PUFAs (n3-LCPUFAs) serve as precursors for bioactive jasmonates. However, how omega-3 fatty acid desaturases (n3-FADs) contribute to the production of n3-PUFAs and jasmonates remain unexplored. Here, we study the n3-FAD-dependent jasmonate signaling in the liverwort M. polymorpha. We found that MpFAD3, localized to the endoplasmic reticulum, prefers omega-6 (n6) arachidonic acid (ARA, 20:4n6) as the substrate thus controlling the biosynthesis of n3-LCPUFA derived jasmonates. Conversely, MpFAD7, found in the chloroplast, takes hexadecadienoic acid (HDA, 16:2n6) and linoleic acid (LA, 18:2n6) as substrates more efficiently and regulates the production of n3-PUFA derived jasmonates. Certain promiscuity was observed for these enzymes as evidenced by increased n6-(LC)PUFA levels in the doble Mpfad3fad7 mutant compared to single mutant lines individually. Mpfad7 and Mpfad3fad7 plants were more resistant to Fusarium oxysporum infection compared to wildtype control, suggesting a prominent role for n3-PUFA derived jasmonates in regulating defenses against necrotrophic pathogens. Remarkably, despite the lack of jasmonate-dependent anti-herbivore defenses in Mpfad3fad7 plants, Spodoptera exigua caterpillars performed equally badly on this double mutant and on wildtype plants, which states the dietary importance of essential n3-(LC)PUFAs for the caterpillar. Our results suggest that two conserved but specialized n3-FADs control n3-(LC)PUFA and jasmonate biosynthesis in Marchantia, and that food quality, namely the type and ratio of n3-(LC)PUFAs, contributes to insect performance in addition to jasmonate-dependent plant defenses.","dates":{"publication":"2026/07/22"},"accession":"GSE305196","cross_references":{"GSM":["GSM9164500","GSM9164511","GSM9164501","GSM9164510","GSM9164504","GSM9164505","GSM9164502","GSM9164503","GSM9164508","GSM9164509","GSM9164506","GSM9164507"],"GPL":["28764"],"GSE":["305196"],"taxon":["Marchantia polymorpha"],"PMID":["[41952316]"]}}