{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305239/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305239"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic profiling of STING-agonist-sensitive and resistant glioblastoma cells under acute diABZI stimulation and chronic selection","description":"Glioblastoma (GBM) exhibits profound therapeutic resistance and an immunosuppressive tumor microenvironment. The cGAS–STING pathway is an attractive target, yet sustained pathway activation can trigger adaptive escape. To define tumor-intrinsic adaptations to STING agonists, we generated an isogenic model using H4 GBM cells: (i) parental H4 treated with vehicle (DMSO, 6 h), (ii) parental H4 challenged acutely with diABZI (1 uM, 6 h), and (iii) a resistant subline (H4-R) derived by chronic diABZI dose escalation (up to 20 uM for >2 months) and harvested drug-free. Poly(A) RNA-seq (NovaSeq 6000, paired-end 150 bp) was performed in biological triplicates. Acute STING activation in parental cells induced a stereotypic interferon/chemokine program centered on CXCL10 together with apoptosis-related genes, whereas H4-R showed suppression of DNA-sensing/STING outputs and a shift toward hypoxia/ECM signatures. In functional assays, CXCL10 neutralization reduced STING-agonist efficacy, while recombinant CXCL10 restored responses. Processed results are provided in GEO; raw FASTQ files are in SRA under the linked BioProject. This dataset enables reuse for benchmarking STING biology, chemokine signaling, and resistance mechanisms in GBM.","dates":{"publication":"2026/09/01"},"accession":"GSE305239","cross_references":{"GSM":["GSM9166554","GSM9166553","GSM9166552","GSM9166558","GSM9166557","GSM9166556","GSM9166555","GSM9166559","GSM9166560"],"GPL":["24676"],"GSE":["305239"],"taxon":["Homo sapiens"]}}