GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305453/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305453GEOGSEfalseIn vivo Perturb-seq uncovers key obstacles to heart repair and regeneration through direct reprogrammingWe investigated the mechanism underlying Calr suppression-enhanced cardiac reprogramming. As CALR functions as an ER-resident chaperone and Ca2+-binding protein, with Ca2+ acting as a second messenger in fate-determining pathways, particularly cardiogenesis we performed RNA-seq on MGTMyoS-induced MICFs transduced with shCalr or shNT at days 7 and 14 in vitro2026/03/21GSE305453GSM9177278GSM9177279GSM9177276GSM9177287GSM9177277GSM9177281GSM9177282GSM9177280GSM9177285GSM9177274GSM9177275GSM9177286GSM9177283GSM9177273GSM917728424247305453Mus musculus