{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305729/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305729"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"DNA origami vaccines program antigen-focused germinal centers","description":"Priming rare subdominant precursor B cells in germinal centers (GCs) is a central goal of vaccination to generate broadly neutralizing antibodies (bnAbs) against HIV. Multivalent immunogen display on protein nanoparticles is a well-established method to promote such responses. However, these nanoparticles generate scaffold-specific B cells that could theoretically limit bnAb precursor expansion in GCs. We rationally designed DNA origami-based virus-like particles (VLPs) displaying a germline-targeting HIV Env immunogen, which elicited no scaffold-specific antibody responses and increased the expansion of epitope-specific GC B cells relative to off-target B cells >10-fold compared with a state-of-the-art clinical protein nanoparticle, which failed to expand bnAb precursors in these conditions. Our results demonstrate that minimizing off-target responses enhances bnAb priming and support DNA-VLPs as a potent vaccine platform.","dates":{"publication":"2026/08/12"},"accession":"GSE305729","cross_references":{"GSM":["GSM9183615","GSM9183616","GSM9183613","GSM9183614","GSM9183622","GSM9183623","GSM9183612","GSM9183620","GSM9183621","GSM9183619","GSM9183617","GSM9183618"],"GPL":["19057"],"GSE":["305729"],"taxon":["Mus musculus"],"PMID":["[41643005]"]}}