GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305729/primary200OKTranscriptomicsMus musculus OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305729GEOGSEfalseDNA origami vaccines program antigen-focused germinal centersPriming rare subdominant precursor B cells in germinal centers (GCs) is a central goal of vaccination to generate broadly neutralizing antibodies (bnAbs) against HIV. Multivalent immunogen display on protein nanoparticles is a well-established method to promote such responses. However, these nanoparticles generate scaffold-specific B cells that could theoretically limit bnAb precursor expansion in GCs. We rationally designed DNA origami-based virus-like particles (VLPs) displaying a germline-targeting HIV Env immunogen, which elicited no scaffold-specific antibody responses and increased the expansion of epitope-specific GC B cells relative to off-target B cells >10-fold compared with a state-of-the-art clinical protein nanoparticle, which failed to expand bnAb precursors in these conditions. Our results demonstrate that minimizing off-target responses enhances bnAb priming and support DNA-VLPs as a potent vaccine platform.2026/08/12GSE305729GSM9183615GSM9183616GSM9183613GSM9183614GSM9183622GSM9183612GSM9183623GSM9183620GSM9183621GSM9183619GSM9183617GSM918361819057305729Mus musculus[41643005]