GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305875/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305875GEOGSEfalseNeonatal overfeeding accelerates age-related sarcopenia and metabolic disorders through lifelong gut mucosa and microbiota programmingEarly-life nutrition shapes lifelong health, yet the long-term consequences of childhood overnutrition, particularly on musculoskeletal aging, remain unclear. To address this gap, we employed an established neonatal overfeeding model by reducing litter size, assigning litters to either 4 pups per dam (Overfed group) or 8 pups per dam (Normal-fed control group). To directly examine whether microbiota alterations mediate neonatal overnutrition-induced acceleration of sarcopenia, we performed fecal microbiota transplantation (FMT) experiments. We transplanted microbiota from either aged normal-fed or overfed donor mice into adult recipients (rNormal and rOver groups) maintained on a high-fat diet (HFD) for 20 weeks to accelerate the onset of metabolic and muscular dysfunction. These findings reveal a developmental origin of age-related sarcopenia and define a gut-muscle axis linking childhood overnutrition to muscle aging.2026/08/27GSE305875GSM9186560GSM9186561GSM9186572GSM9186573GSM9186562GSM9186563GSM9186574GSM9186564GSM9186565GSM9186566GSM9186567GSM9186570GSM9186568GSM918656924247305875Mus musculus