{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE306nnn/GSE306051/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306051"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Comprehensive analysis of the effects of mitomycin C on human conjunctival fibroblasts","description":"RNA-Seq analysis was conducted to investigate the effects of mitomycin C (MMC) on human conjunctival fibroblasts (HConFs). Significant changes in gene expression were observed at 3 hours, 3 days, and 14 days after MMC treatment, with 322, 4,231, and 5,095 genes significantly altered compared to controls, respectively. Enrichment analysis revealed a notable upregulation of genes involved in apoptosis and the p53 pathway, as well as a significant downregulation of genes associated with cell proliferation such as those involved in the G2M checkpoint and E2F targets. Furthermore, the expression of genes related to the senescence-associated secretory phenotype, including FGF2, CCL2, and CCL7, was also increased, suggesting that MMC induces cellular senescence in HConFs as indicated by RNA-Seq analysis.","dates":{"publication":"2026/08/26"},"accession":"GSE306051","cross_references":{"GSM":["GSM9192127","GSM9192128","GSM9192118","GSM9192129","GSM9192119","GSM9192123","GSM9192124","GSM9192125","GSM9192126","GSM9192120","GSM9192121","GSM9192122"],"GPL":["18573"],"GSE":["306051"],"taxon":["Homo sapiens"],"PMID":["[42625400]"]}}