GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE306nnn/GSE306141/primaryOK200GenomicsHomo sapiensGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306141GEOGSEfalseA side-by-side comparison of commercial CUT&RUN kitsIntroduction: CUT&RUN (Cleavage Under Targets and Release Using Nuclease) followed by next-generation sequencing is a powerful method increasingly used in place of ChIP-seq to profile genome-wide protein-DNA interactions including those of transcription factors, histone modifications and chromatin remodelers. Although the choice of antibody is well known to affect data quality, we found that enrichment chemistry and protocol significantly affect data quality as well. Methods: In this study, we evaluated the performance of three commercially available CUT&RUN kits (Active Motif - ChIC/CUT&RUN Assay Kit, Cell Signaling Technology (CST) – CUT&RUN Assay Kit, EpiCypher - CUTANA™ ChIC/CUT&RUN Kit) on two target histone marks to represent sharp peaks (H3K4me3) and broad domains (H3K27me3). ATAC-seq data was integrated to assess the accuracy of peaks generated with each CUT&RUN kit.2026/05/15GSE306141GSM919398324676306141Homo sapiens[42088458]