{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE306nnn/GSE306148/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306148"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Fucci-G1 insertion partially disrupts Shh expression","description":"The Fucci mouse lines are valuable tools for tracing cell cycle stages during development and in postnatal tissues; however, the insertion loci for the transgenes remain unidentified. Here, we report the genomic insertion sites of both the Fucci-G1 and -S/G2/M transgenes, identified through whole genome sequencing. Interestingly, the Fucci-G1 transgene is located proximal to the Shh lung gut enhancer (SLGE) on chromosome 5. Homozygous Fucci-G1 embryos survive until embryonic day 18.5 (E18.5), exhibiting craniofacial and foregut-related defects, but are perinatal lethal. Expression of Shh and canonical targets are down-regulated in the pharyngeal arches of E11.5 homozygous Fucci-G1 embryos. Furthermore, in situ hybridization analysis showed diminished Shh expression in the pharyngeal ectoderm and foregut endoderm, consistent with disruptions to SLGE activity. Our results indicate that heterozygous Fucci-G1 mice are suitable for cell cycle studies; however, we caution potential genetic interference when investigating SHH-related pathways.","dates":{"publication":"2026/06/16"},"accession":"GSE306148","cross_references":{"GSM":["GSM9194092","GSM9194081","GSM9194091","GSM9194080","GSM9194083","GSM9194082","GSM9194093","GSM9194090","GSM9194089","GSM9194078","GSM9194077","GSM9194088","GSM9194079","GSM9194085","GSM9194084","GSM9194087","GSM9194076","GSM9194086"],"GPL":["34290"],"GSE":["306148"],"taxon":["Mus musculus"]}}